Coding
acdS

Part:BBa_K4392001

Designed by: Vikram Venugopal   Group: iGEM22_IISER-Pune-India   (2022-10-01)


ACC deaminase from Pseudomonas fluorescens

This part codes for the ACC deaminase (acdS) gene from Pseudomonas fluorescens FY32. ACC deaminase breaks down 1-aminocyclopropane-1-carboxylic acid into alpha ketobutyrate and ammonia. The acdS enzyme can break down ACC in bacteria or plants. ACC is the precursor of ethylene, so its breakdown helps to prevent harmful effects caused by ethylene stress responses in plants. A BioBrick prefix and suffix flag this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 870
    Illegal XhoI site found at 187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 975
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 210


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Categories
Parameters
None